Leukemia Methods and Protocols - So, Chi Wai Eric.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
An Overview: From Discovery of Candidate Mutations to Disease Modeling and Transformation Mechanisms of Acute Leukemia -- Backtracking of Leukemic Clones to Birth -- Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia -- Specialized Fluorescence In Situ Hybridization (FISH) Techniques for Leukaemia Research -- LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene -- Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences -- MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR -- Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome -- Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer -- Retroviral/Lentiviral Transduction and Transformation Assay -- Gene Expression Profiling of Leukemia Stem Cells -- Humanized Model to Study Leukemic Stem Cells -- Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells via Retroviral Transduction -- Ex Vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human Acute Myeloid Leukemia Stem Cells -- Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry -- In Vitro Differentiation of Embryonic Stem Cells as a Model of Early Hematopoietic Development -- Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche -- Identification of Protein Interaction Partners by the Yeast Two-Hybrid System -- Complete Array of HOX Gene Expression by RQ-PCR -- Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias -- Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated Proteins.
Keywords: Cancer research; Oncology .; Laboratory medicine; Cancer Research; Oncology; Laboratory Medicine