Protein Targeting Protocols - van der Giezen, Mark.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2007)
Bacterial Systems -- Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching -- Membrane Protein Insertion and Secretion in Bacteria -- Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria -- Periplasmic Targeting of Recombinant Proteins in Escherichia coli -- In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export -- Eukaryotic Systems -- Nuclear Import Properties of the Sex-Determining Factor SRY -- The Mitochondrial Machinery for Import of Precursor Proteins -- A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells -- In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria -- Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa -- A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70 -- The Chloroplast Protein Import Machinery: A Review -- Import of Plastid Precursor Proteins Into Pea Chloroplasts -- Protein Targeting in ''Secondary'' or ''Complex'' Chloroplasts -- Protein Trafficking to the Complex Chloroplasts of Euglena -- Fluorescent Protein Fusions for Protein Localization in Plants -- Genetic Transformation -- Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening -- Trafficking Through the Early Secretory Pathway of Mammalian Cells -- Studying Protein Export From the Endoplasmic Reticulum in Plants -- Imaging the Golgi Apparatus in Living Mitotic Cells -- Evaluating Yeast Biosynthetic Vacuolar Transport -- Targeting to Lysosomes in Mammalian Cells -- Monitoring Autophagy in Yeast -- Protein Targeting to Yeast Peroxisomes -- Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins -- General Methods -- Intracellular Protein Localization by Immunoelectron Microscopy -- Separation of Proteins by Blue Native Electrophoresis -- Computational Prediction of Subcellular Localization -- Phylogenetic Analysis to Uncover Organellar Origins of Nuclear-Encoded Genes.
Keywords: Proteins .; Cell biology; Human genetics; Protein Science; Cell Biology; Human Genetics