Neutrophil Methods and Protocols - Quinn, Mark T.; DeLeo, Frank R.; Bokoch, Gary M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2007)
Neutrophils: An Overview -- The Role of Neutrophils in the Immune System -- Neutrophil Isolation and Subcellular Fractionation -- Isolation of Human Neutrophils From Venous Blood -- Neutrophil Isolation From Nonhuman Species -- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers -- Biochemistry, Biology, and Signal Transduction of Neutrophils -- Rac and Rap GTPase Activation Assays -- Measurement of Phospholipid Metabolism in Intact Neutrophils -- Analysis of Protein Phosphorylation in Human Neutrophils -- Mitogen-Activated Protein Kinase Assays -- Transduction of Proteins into Intact Neutrophils -- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils -- Analysis of Electrophysiological Properties and Responses of Neutrophils -- Analysis of Neutrophil Apoptosis -- Neutrophil Adhesion and Chemotaxis -- Optical and Fluorescence Detection of Neutrophil Integrin Activation -- Analysis of Neutrophil Polarization and Chemotaxis -- Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils -- Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow -- Model Systems to Investigate Neutrophil Adhesion and Chemotaxis -- Neutrophil Phagocytosis and Bactericidal Activity -- Immunofluorescence and Confocal Microscopy of Neutrophils -- Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy -- Analysis of Neutrophil Membrane Traffic During Phagocytosis -- Analysis of Neutrophil Bactericidal Activity -- A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions -- Nadph Oxidase and Production of Reactive Oxygen Species -- Measurement of Respiratory Burst Products Generated by Professional Phagocytes -- The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function -- Cell-Free Assays -- Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics -- Analysis of Neutrophil Gene Expression and Transcription Factors -- Genome-Scale Transcript Analyses in Human Neutrophils -- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR -- Detection of Intact Transcription Factors in Human Neutrophils -- Neutrophil Defects and Diagnosis -- Disorders of Neutrophil Function -- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders -- Diagnostic Assays for Myeloperoxidase Deficiency.
Keywords: Immunology; Cell biology; Human genetics; Immunology; Cell Biology; Human Genetics