Transgenic Maize Methods and Protocols - Scott, M. Paul.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Design of Gene Constructs for Transgenic Maize -- Transformation Methods -- Transient Expression of GFP in Immature Seed Tissues -- Biolistic Gun-Mediated Maize Genetic Transformation -- Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus -- Whiskers-Mediated Maize Transformation -- Transgenic Maize in Research -- Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines -- Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression -- Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites -- Analysis of Transgenic Plants -- Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize -- Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds -- Determination of Transgene Copy Number by Real-Time Quantitative PCR -- Breeding with Transgenes -- Herbicide Resistance Screening Assay -- Characterizing Transgene Inheritance -- Backcross Breeding.
Keywords: Agriculture; Plant science; Botany; Plant genetics; Transgenic organisms; Agriculture; Plant Sciences; Plant Genetics and Genomics; Transgenics