Methods in Membrane Lipids - Dopico, Alex.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2007)
A Glance at the Structural and Functional Diversity of Membrane Lipids -- Membrane Lipid Polymorphism -- Acrylodan-Labeled Intestinal Fatty Acid-Binding Protein to Measure Concentrations of Unbound Fatty Acids -- Measuring Molecular Order and Orientation Using Coherent Anti-Stokes Raman Scattering Microscopy -- Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scattering -- Nuclear Magnetic Resonance Investigation of Oriented Lipid Membranes -- Molecular Dynamics Simulations as a Complement to Nuclear Magnetic Resonance and X-Ray Diffraction Measurements -- Use of Inverse Theory Algorithms in the Analysis of Biomembrane NMR Data -- Statistical Thermodynamics Through Computer Simulation to Characterize Phospholipid Interactions in Membranes -- Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes -- Fluorescence Detection of Signs of Sterol Superlattice Formation in Lipid Membranes -- Differential Scanning Calorimetry in the Study of Lipid Phase Transitions in Model and Biological Membranes -- Pressure Perturbation Calorimetry -- Fourier Transform Infrared Spectroscopy in the Study of Lipid Phase Transitions in Model and Biological Membranes -- Optical Dynamometry to Study Phase Transitions in Lipid Membranes -- Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes -- Measurement of Lateral Diffusion Rates in Membranes by Pulsed Magnetic Field Gradient, Magic Angle Spinning-Proton Nuclear Magnetic Resonance -- Using Fluorescence Recovery After Photobleaching to Measure Lipid Diffusion in Membranes -- Single-Molecule Fluorescence Microscopy to Determine Phospholipid Lateral Diffusion -- Modeling 2D and 3D Diffusion -- Measurement of Water and Solute Permeability by Stopped-Flow Fluorimetry -- Fluorescence Microscopy to Study Pressure Between Lipids in Giant Unilamellar Vesicles -- X-Ray Scattering and Solid-State Deuterium Nuclear Magnetic Resonance Probes of Structural Fluctuations in Lipid Membranes -- Determination of Lipid Spontaneous Curvature From X-Ray Examinations of Inverted Hexagonal Phases -- Shape Analysis of Giant Vesicles With Fluid Phase Coexistence by Laser Scanning Microscopy to Determine Curvature, Bending Elasticity, and Line Tension -- Laser Tweezer Deformation of Giant Unilamellar Vesicles -- Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress -- Micropipet Aspiration for Measuring Elastic Properties of Lipid Bilayers -- Langmuir Films to Determine Lateral Surface Pressure on Lipid Segregation -- Detergent and Detergent-Free Methods to Define Lipid Rafts and Caveolae -- Near-Field Scanning Optical Microscopy to Identify Membrane Microdomains -- Fluorescence Microscopy to Study Domains in Supported Lipid Bilayers -- Fluorescence Resonance Energy Transfer to Characterize Cholesterol-Induced Domains -- Lipid Domains in Supported Lipid Bilayer for Atomic Force Microscopy -- Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers -- Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions -- Single-Molecule Methods for Monitoring Changes in Bilayer Elastic Properties -- Ion-Channel Reconstitution -- The Use of Differential Scanning Calorimetry to Study Drug-Membrane Interactions -- Atomic Force Microscopy to Study Interacting Forces in Phospholipid Bilayers Containing General Anesthetics.
Keywords: Biochemistry; Cell biology; Molecular biology; Biochemistry, general; Cell Biology; Molecular Medicine