Mitochondrial DNA Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 2nd ed. 2009. |
| Colección: | Methods in Molecular Biology,
554 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-59745-521-3 |
Tabla de Contenidos:
- Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly
- Biochemical Isolation of mtDNA Nucleoids from Animal Cells
- Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
- Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins: DNA Polymerase ? and Mitochondrial Single-Stranded DNA-Binding Protein
- Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
- Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein
- Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
- Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Helicase Twinkle
- Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
- Methods for Studying Mitochondrial Transcription Termination with Isolated Components
- Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes
- Mitochondrial DNA Damage and Repair
- Reactive Oxygen Species Production by Mitochondria
- Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
- Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method
- In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria
- Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction
- Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nuclear, Oxidative DNA Damage
- Mitochondrial DNA Mutations
- Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae
- Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis
- Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease
- Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells
- Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR
- Measuring DNA Precursor Pools in Mitochondria
- Establishment of Human Cell Lines Lacking Mitochondrial DNA.