Potassium Channels Methods and Protocols - Lippiat, Jonathan D.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Methods For Studying Potassium Channel Transcription And Mrna Splicing -- Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation -- Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries -- Cloning of Potassium Channel Splice Variants from Tissues and Cells -- Methods for Studying the Cell Biology of Potassium Channel Proteins -- Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels -- Investigation of KATP Channel Endocytosis by Immunofluorescence -- Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting -- Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts -- Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein-Lipid Overlay Assay -- Protein Complex Analysis of Native Brain Potassium Channels by Proteomics -- Electrophysiological Techniques For The Study Of Potassium Channel Function -- Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique -- Whole-Cell Recording Using the Perforated Patch Clamp Technique -- Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration -- Planar Patch Clamp: Advances in Electrophysiology -- Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers -- Optical Techniques For The Study Of Potassium Channel Function And Interactions -- Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly -- The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels -- The Voltage-Clamp Fluorometry Technique -- Methods For Studying Potassium Channelopathies And Pharmacological Modulators -- Identification of Mutations in the Kir6.2 Subunit of the KATP Channel -- Modulation of Potassium Ion Channel Proteins Utilising Antibodies -- Fluorescence-Based Tl+?Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+?Activated K+ Channel Modulators -- Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties -- Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique.
Keywords: Pharmacology; Cell biology; Cell membranes .; Pharmacology/Toxicology.; Cell Biology; Membrane Biology