Protein Blotting and Detection Methods and Protocols - Kurien, Biji T.; Scofield, R. Hal.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Origins of Protein Blotting -- Western Blotting: Remembrance of Things Past -- to Protein Blotting -- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis -- Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support -- Tryptic Peptide Purification Using Polyvinylidene Difluoride Membrane for Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry -- Affinity Immunoblotting for Analysis of Antibody Clonotype Distribution in a Lupus Patient Developing Anti-Ro 60 Over Time -- Nonelectrophoretic Bidirectional Transfer of a Single SDS-PAGE Gel with Multiple Antigens to Obtain 12 Immunoblots -- Slice Blotting -- Localizing Proteins by Tissue Printing -- Dot-Immunobinding Assay -- Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes After Agarose-Gel Electrophoresis Separation -- Quantitative computerized western blotting -- Cationic electrophoresis and eastern blotting -- A miniaturized blotting system for simultaneous detecting of differentautoantibodies -- Transfer and Multiplex Immunoblotting of a Paraffin Embedded Tissue -- Multistrip Western Blotting to Increase Quantitative Data Output -- Western Blotting/Edman Sequencing using PVDF Membrane -- Blotting from PhastGel to Membranes by Ultrasound -- Ultrarapid Electrophoretic Transfer of High and Low Molecular Weight Proteins Using Heat -- Strip Immunoblotting of Multiple Antigenic Peptides to Nitrocellulose Membrane -- Affinity Purification of Autoantibodies from an Antigen Strip Excised from a Nitrocellulose Protein Blot -- Double-Blotting: A Solution to the Problem of Nonspecific Binding of Secondary Antibodies in Immunoblotting Procedures -- Efficient Electroblotting of Very Large Proteins Using a Vertical Agarose Electrophoresis System -- Semi-Dry Protein Transfer and Immunodetection of P-Selectin Using an Antibody to its C-terminal Tag -- The Multiple Antigen Blot Assay: A Simple, Versatile and Multipurpose Immunoenzymatic Technique -- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates -- Immunoprecipitation-Western Blot for Proteins of Low Abundance -- Native Electrophoresis and Western Blot Analysis: Method and Applications -- Grid-Immunoblotting -- Sequential Use of Immunoblots for Characterization of Autoantibody Specificities -- Renaturation of Recombinant Ro 60 Autoantigen by Calcium Ions on PVDF Membrane -- Intermittent Microwave Irradiation Facilitates Antigen-Antibody Reaction in Western Blot Analysis -- Detection of Protein-Protein Interactions by Far-Western Blotting -- On-Membrane Tryptic Digestion of Proteins for Mass Spectrometry Analysis -- Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay -- ELISPOT Assay on Membrane Microplates -- A Brief Review of Other Notable Protein Blotting Methods -- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore -- Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation -- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting -- Whole and Strip Nitrocellulose Membrane as well as a New-Line-Immunoblotting ofAntigen Using the Chemiluminescence Technique -- Protein Stains to Detect Antigen on Membranes -- Use of non-radioactive detection method for North- and South-Western Blot -- Immunoblotting using Radiolabeled Reagents for Detection -- Detection of Protein Carbonyls by Means of Biotin Hydrazide-Streptavidin Affinity Methods -- "Rainbow" Western Blotting -- Multiple antigen detection (MAD) western blotting -- Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2- -- Detection of La/SS-B by Western Blot using Nanogold-Tagged Antibodies andSilver Enhancement -- Imaging Systems for Westerns: Chemiluminescence vs. Infrared Detection -- A Fluorescent Codetection System for Immunoblotting and Proteomics Through ECL-Plex and CyDye Labeling -- Visualization of Unstained Protein Bands on PVDF -- Improved Protein Detection Using Cold Microwave Technology -- TLC Blot (Far-Eastern Blot) and Its Applications -- A Brief Review of Other Notable Protein Detection Methods on Blots -- Sending Secret Messages on Nitrocellulose Membrane and the Use of a Molecular Pen for Orientation in ECL Membrane Assays.
Keywords: Proteins .; Proteomics; Protein Science; Proteomics