Transgenesis Techniques Principles and Protocols - Cartwright, Elizabeth J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Transgenesis in Various Model Systems -- Transgenesis in Drosophila melanogaster -- Transgenesis in Caenorhabditis elegans -- Transgenesis in Zebrafish with the Tol2 Transposon System -- Generation of Transgenic Frogs -- Pronuclear DNA Injection for the Production of Transgenic Rats -- Transgenesis in the Mouse -- Cell-Type-Specific Transgenesis in the Mouse -- Transgene Design and Delivery into the Mouse Genome: Keys to Success -- Overexpression Transgenesis in Mouse: Pronuclear Injection -- Gene-Targeting Vectors -- Gene Trap: Knockout on the Fast Lane -- Culture of Murine Embryonic Stem Cells -- Targeting Embryonic Stem Cells -- Generation of Chimeras by Microinjection -- Generation of Chimeras by Morula Aggregation -- Surgical Techniques for the Generation of Mutant Mice -- Site-Specific Recombinases for Manipulation of the Mouse Genome -- Cre Transgenic Mouse Lines -- Large-Scale Mouse Mutagenesis -- Dedicated Mouse Production and Husbandry -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part II: Recovery and Distribution of Conserved Mutant Strains.
Keywords: Genetic engineering; Human genetics; Animal genetics; Transgenic organisms; Genetic Engineering; Human Genetics; Animal Genetics and Genomics; Transgenics