Mesenchymal Stem Cells Methods and Protocols - Prockop, Darwin J.; Phinney, Donald G.; Bunnell, Bruce A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Isolation of Human Multipotential Stromal Cells from Bone Marrow and Adipose Tissue -- Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs) -- Mesenchymal Stem Cells from Adult Bone Marrow -- A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells -- Adipose-Derived Stem Cells -- Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens -- Manipulation of Human Multipotential Stromal Cells -- Colony Forming Unit Assays for MSCs -- Differentiation and Characterization of Human MSCs -- Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis, and Experimentation -- Gene Expression Analysis at the Single Cell Level Using the Human Bone Marrow Stromal Cell as a Model: Sample Preparation Methods -- Assays of MSCs with Microarrays -- Gene Delivery to Mesenchymal Stem Cells -- Isolation and Characterization of Murine Multipotential Stromal Cells from Bone Marrow -- Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion.
Keywords: Cell biology; Molecular biology; Immunology; Cell Biology; Molecular Medicine; Immunology