Molecular Biomethods Handbook - Walker, John M.; Rapley, Ralph; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Nucleic Acid Methods -- The Manipulation of Nucleic Acids -- Restriction Enzymes -- Principles and Medical Applications of the Polymerase Chain Reaction -- Probe Design, Production, and Applications -- Southern Blotting as a Diagnostic Method -- Capillary Electrophoresis of DNA -- Denaturing High-Performance Liquid Chromatography (DHPLC) for Nucleic Acid Analysis -- Denaturing Gradient Gel Electrophoresis (DGGE) -- Single Strand Conformation Polymorphism (SSCP) Analysis -- Randomly Amplified Polymorphic DNA (RAPD) -- Quantification of mRNA Using Real Time RT-PCR -- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing -- Multiplex Amplifiable Probe Hybridization (MAPH) -- Gene Expression Profiling -- Comparative Genomic Hybridization in Clinical and Medical Research -- Subtractive Hybridization -- Fluorescence In Situ Hybridization -- Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice -- cDNA Microarrays -- Mapping Techniques -- Single Nucleotide Polymorphisms -- Gene/Protein Sequence Analysis -- Protein and Cell Methods -- Protein Electrophoresis -- Protein Blotting -- Capillary Electrophoresis of Proteins -- Autoradiography and Fluorography -- Mass Spectrometry of Proteins and Peptides -- Post-Translational Modif ications of Proteins -- Protein Microarray Technology -- Protein-Protein Interactions -- Glycoprotein Analysis -- Solid-Phase Peptide Synthesis -- Monoclonal Antibodies -- Antibody Phage Display -- Protein Engineering -- Directed Protein Evolution -- Enzyme Linked Immunosorbent Assay (ELISA) -- Epitope Mapping -- Quantum Dots -- Ion-Exchange Chromatography -- Size-Exclusion Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- High Performance Liquid Chromatography (HPLC) of Peptides and Proteins -- Amino Acid Analysis -- Surface Plasmon Resonance -- Macromolecular Crystallography -- Microchip Devices for Bioanalysis -- Mammalian Cell Culture -- Plant Tissue Culture -- Stem Cells and Regenerative Medicine -- Cryopreservation -- Magnetic Resonance Imaging -- Electron Microscopy -- Confocal Microscopy -- Laser Microdissection -- Biomedical Uses of Flow Cytometry -- Immunomicroscopy -- In Situ Hybridization -- High Throughput Screening.
Keywords: Molecular biology; Molecular Medicine