Molecular Endocrinology Methods and Protocols - Park-Sarge, Ok-Kyong.; Curry, Jr., Thomas E.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Determining the Affinity of Hormone?Receptor Interaction -- Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques -- Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and Their Functional Consequences at the Hormone-Receptor Interaction Level -- Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane -- Gene Expression Profiling in the Aging Ovary -- Genomics Analysis: Endometrium -- Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization -- Adenoviral Gene Transfer into Isolated Pancreatic Islets -- Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes -- Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines -- Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells -- Use of Reporter Genes to Study Promoters of the Androgen Receptor -- Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor ? -- Chromosome-Wide Analysis of Protein Binding and Modifications -- Genome-Wide Analysis for Protein?DNA Interaction: ChIP-Chip -- Detection of ER?-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer -- Detection of Proteins Sumoylated In Vivo and In Vitro -- Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE) -- Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis -- Reporter Mice for the Study of Intracellular Receptor Activity -- Real-Time Non-invasive Imaging of ES Cell-Derived Insulin Producing Cells -- Transgenic Mouse Technology: Principles and Methods -- Breast Tumor-Initiating Cells Isolated from Patient Core Biopsies for Study of Hormone Action -- Markers of Oxidative Stress and Sperm Chromatin Integrity -- Planning and Executing a Genome Wide Association Study (GWAS).
Keywords: Endocrinology .; Human physiology; Molecular biology; Endocrinology; Human Physiology; Molecular Medicine