Experiments in Molecular Biology - Slater, Robert J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1986)
DNA Isolation and Transformation in Escherichia coli -- Isolation of Plasmid DNA -- Restriction Endonuclease Digestion and Agarose Gel Electrophoresis of DNA -- Restriction Site Mapping -- Southern Blotting -- Preparative Electrophoresis -- DNA Ligation and Escherichia coli Transformation -- Detection of Plasmid by Colony Hybridization -- Microisolation and Microanalysis of Plasmid DNA -- Isopycnic Centrifugation of DNA -- Purification of Plant Ribosomal DNA -- The Extraction and Affinity Chromatography of Messenger RNA -- Agarose Gel Electrophoresis of RNA -- In Vitro Protein Synthesis -- SDS Polyacrylamide Gel Electrophoresis of Proteins -- Isoelectric Focusing (IEF) of Proteins in Thin-Layer Polyacrylamide Gels -- Protein (Western) Blotting with Immunodetection -- Thin-Layer Gel Filtration -- The Dansyl-Edman Method for Peptide Sequencing -- The Double Immunodiffusion Technique -- Immunoelectrophoretic Techniques -- The Time Course of ?-Galactosidase Induction in Escherichia coli -- Experiments on Enzyme Induction in the Unicellular Green Alga Chlorella fusca -- Yeast Protoplast Fusion -- The Ames Test.
Keywords: Cell biology; Cell Biology