The Nucleus Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging - Hancock, Ronald; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Physical and Mechanical Properties -- Physical Properties of the Nucleus Studied by Micropipette Aspiration -- Mechanical Properties of Interphase Nuclei Probed by Cellular Strain Application -- Chromatin and Transcription -- Gene Expression in Polytene Nuclei -- Electron Microscope Visualization of RNA Transcription and Processing in Saccharomyces cerevisiae by Miller Chromatin Spreading -- Combing Genomic DNA for Structural and Functional Studies -- Using Molecular Beacons to Study Dispersal of mRNPs from the Gene Locus -- Mapping Cis- and Trans- Chromatin Interaction Networks Using Chromosome Conformation Capture (3C) -- Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope -- Using Cells Encapsulated in Agarose Microbeads to Analyse Nuclear Structure and Functions -- The Nuclear Envelope -- Investigation of Nuclear Envelope Structure and Passive Permeability -- Reconstitution of Nuclear Import in Permeabilized Cells -- Nuclear Envelope Formation In Vitro: A Sea Urchin Egg Cell-Free System -- Modifications of Nuclear Proteins -- Detection and Analysis of (O-linked ?-N-Acetylglucosamine)-Modified Proteins -- Detection of Sumoylated Proteins -- Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Damage -- Purification and Analysis of Variant and Modified Histones Using 2D PAGE -- Quantification of Redox Conditions in the Nucleus -- Protein Dynamics in the Nucleus -- Fluorescence Correlation Spectroscopy to Assess the Mobility of Nuclear Proteins -- Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics -- Fluorescence Recovery After Photobleaching (FRAP) to Study Nuclear Protein Dynamics in Living Cells -- Imaging Methods -- Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus -- Visualisation of RNA by Electron Microscopic In Situ Hybridisation -- Electron Spectroscopic Imaging of the Nuclear Landscape -- Cryoelectron Microscopy of Vitreous Sections: A Step Further Towards the Native State.
Keywords: Biochemistry; Cell biology; Cell physiology; Biochemistry, general; Cell Biology; Cell Physiology