Phospho-Proteomics Methods and Protocols - de Graauw, Marjo; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Protein Phosphorylation and 2D Gel Electrophoresis -- A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling -- Differential Phosphoprotein Labelling (DIPPL) Using 32P and 33P -- Enrichment of Phosphoproteins and Peptides -- Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry -- Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry -- The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests -- Enrichment and Separation of Mono- and Multiply Phosphorylated Peptides Using Sequential Elution from IMAC Prior to Mass Spectrometric Analysis -- Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation -- Hydrophilic Interaction Chromatography for Fractionation and Enrichment of the Phosphoproteome -- SILAC for Global Phosphoproteomic Analysis -- Quantitative Phospho-proteomics Based on Soluble Nanopolymers -- Profiling the Tyrosine Phosphorylation State Using SH2 Domains -- Phosphoprotein Analysis by Mass Spectrometry -- An Overview of the Qualitative Analysis of Phosphoproteins by Mass Spectrometry -- The Analysis of Phosphoproteomes by Selective Labelling and Advanced Mass Spectrometric Techniques -- On-Line Liquid Chromatography Electron Capture Dissociation for the Characterization of Phosphorylation Sites in Proteins -- Quantification of Protein Phosphorylation by ?LC-ICP-MS -- Reverse-Phase Diagonal Chromatography for Phosphoproteome Research -- Chemical Tagging Strategies for Mass Spectrometry-Based Phospho-proteomics -- Arrays to Study Protein-Phosphorylation -- Antibody Array Platform to Monitor Protein Tyrosine Phosphorylation in Mammalian Cells -- Protein Tyrosine Kinase Characterization Based on Fully Automated Synthesis of (Phospho) Peptide Arrays in Microplates -- Kinome Profiling Using Peptide Arrays in Eukaryotic Cells -- Bioinformatics -- ProMoST: A Tool for Calculating the pI and Molecular Mass of Phosphorylated and Modified Proteins on Two-Dimensional Gels -- Kinase-Specific Prediction of Protein Phosphorylation Sites -- Reconstructing Regulatory Kinase Pathways from Phosphopeptide Data: A Bioinformatics Approach.
Keywords: Proteins .; Proteomics; Protein Science; Proteomics