Reverse Chemical Genetics Methods and Protocols - Koga, Hisashi; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics -- ORFeome Cloning -- Systematic Cloning of an ORFeome Using the Gateway System -- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins -- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study -- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data -- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology -- Modification of cDNA Resources Utilized for Reverse Chemical Genetics -- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources -- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources -- Recombinant Protein Production by a Kaiko-Baculovirus System -- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins -- The Refinement of Established Methods by Recent Innovations -- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine -- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis -- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos -- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins -- Potential Applicable Techniques for Reverse Chemical Genetics -- Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases -- Preparation of Highly Sensitive Protein Array Using Reactive Polymer -- High-Throughput SPR Biosensor -- Drug Discovery Through Functional Screening in the Drosophila Heart -- Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity -- Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety.
Keywords: Human genetics; Medical genetics; Human Genetics; Gene Function