Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols - Till, Bradley J.; Jankowicz-Cieslak, Joanna; Huynh, Owen A.; Beshir, Mayada M.; Laport, Robert G.; Hofinger, Bernhard J.; SpringerLink (Online service) (Cham : Springer International Publishing : Imprint: Springer, 2015)
Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.  .
Keywords: Plant breeding; Biology-Technique.; Nucleic acids; Plant Breeding/Biotechnology.; Biological Techniques; Nucleic Acid Chemistry