Multi-Parametric Live Cell Microscopy of 3D Tissue Models - Dmitriev, Ruslan I.; SpringerLink (Online service) (Cham : Springer International Publishing : Imprint: Springer, 2017)
Current state-of-the-art 3D tissue models and their compatibility with live cell imaging -- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation -- Quantitative live cell FLIM imaging in three dimensions -- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview -- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues -- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture -- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2 -- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell -- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues -- Live cell imaging of viscosity in 3D tumour cell models -- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy -- Raman imaging microscopy for quantitative analysis of biological samples.
Keywords: Regenerative medicine; Tissue engineering; Microscopy; Biomedical engineering; Spectroscopy; Regenerative Medicine/Tissue Engineering; Biological Microscopy; Biomedical Engineering/Biotechnology.; Biomedical Engineering and Bioengineering; Spectroscopy and Microscopy