Methods in Endothelial Cell Biology - Augustin, Hellmut G.; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2004)
I: Isolation and Culture of Endothelial Cells -- 1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial Cells -- 2 Isolation of Mouse Cardiac Microvascular Endothelial Cells -- 3 Isolation, Culture, and Differentiation of Circulating Endothelial Precursor Cells -- 4 Embryonic Stem Cell-Derived Endothelial Cells -- 5 Detection, Isolation, and Culture Of Lymphatic Endothelial Cells -- 6 Three-Dimensional Spheroid Culture of Endothelial Cells -- 7 Immortalization of Endothelial Cells -- 8 Viral Gene Transfer Into Endothelial Cells -- II: Functional Assays in Vitro -- 9 Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress -- 10 Application of Biomechanical Forces on Endothelial Cells -- 11 Endothelial Cell Permeability Assays in Culture -- 12 Three-Dimensional in Vitro Angiogenesis Assays -- 13 Rat Aortic Ring Assay of Angiogenesis -- 14 Endothelial Cell Chemotaxis and Chemokinesis Assays -- 15 FACS Analysis of Endothelial Cells -- 16 Application of the RNA Interference (RNAi) Technology to Angiogenesis Research -- III: Functional Assays in Vivo -- 17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment -- 18 Balloon Denudation of Blood Vessels -- 19 Peripheral Hind Limb Ischemia Models -- 20 In Vivo Matrigel Angiogenesis Assays -- 21 Analysis of Vascular Permeability in vivo -- 22 Validating the Manipulation of Endothelial Cell signaling in Vivo -- 23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis -- 24 Corneal Angiogenesis Assay -- IV: Detection of Endothelial Cells -- 25 Basic Blood Vessel Immunohistochemistry -- 26 Double-Staining Immunohistochemistry of Blood Vessels -- 27 In Situ Hybridization Analysis of Vascular Endothelium -- 28 High-Resolution in Situ Confocal Analysis of Endothelial Cells -- 29 Whole Mount Analysis of the Embryonic Vasculature -- 30 Methods for Visualizing Intact Blood Vessels -- 31 Intravital Multi-Fluorescence Microscopy -- 32 Corrosion Cast Analysis of Blood Vessels -- Suppliers -- Endothelial Cell Biology on the Internet.
Keywords: Cell biology; Human physiology; Molecular biology; Angiology; Cell Biology; Human Physiology; Molecular Medicine; Angiology