Methods of Preparation for Electron Microscopy An Introduction for the Biomedical Sciences - Robinson, David G.; Ehlers, Ulrich; Herken, Rainer; Herrmann, Bernd; Mayer, Frank; Schürmann, Friedrich-Wilhelm.; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1987)
1 An Introduction to Electron Microscopy (EM) -- 1.1 Imaging Methods in Electron Microscopy -- 1.2 Preparation Procedures in TEM -- 1.3 Imaging Problems -- 1.4 Support Films -- 2 Methods for TEM -- 2.1 Fixation, Dehydration and Embedding -- 2.2 Ultramicrotomy -- 2.3 Macromolecular EM -- 2.4 Immunoelectron Microscopy (I EM) -- 2.5 Autoradiography -- 2.6 Freeze (Fracturing) Etching -- 3 Methods for SEM -- 3.1 Conventional Methods of Preparation -- 3.2 Storage of Specimens -- 3.3 Demonstration of Surfaces via Replicas and Casts -- 3.4 Visualization of Internal Surfaces Through Sectioning and Dry-Fracturing (Dry-Cleaving) -- 3.5 Element Analysis -- 4 Evaluation of Micrographs -- 4.1 Morphometry -- 4.2 Averaging and Image Reconstruction -- Appendix: Buffers in Electron Microscopy.
Keywords: Cell biology; Cell Biology