Cell Cycle - Materials and Methods - Pagano, Michele; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1996)
One Techniques for Studying the Mammalian Cell Cycle -- 1 Use of Immunohistochemistry in Cell Cycle Studies -- 2 Immunofluorescence Techniques in Cell Cycle Studies -- 3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes -- 4 Techniques for Monitoring Cell Cycle Phases -- 5 Centrifugal Elutriation -- 6 Synchronization of Mammalian Cells -- 7 Microinjection and Electroporation of Macromolecules into Live Cells -- 8 Stable and Transient Transfections in Cell Cycle Studies -- 9 Quantification of Premature and Normal Mitosis -- Two Techniques for Studying the Xenopus Cell Cycle -- 10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies -- Three Techniques to Study the Yeast Cell Cycle -- 11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe -- 12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe -- Four Techniques for Studying the Cell Cycle of Any Species -- 13 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands -- 14 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases -- 15 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Pichia pastoris -- 16 SV40 Based in Vitro DNA Replication Assay -- 17 From Sequence to Function-Structural Motifs of Cell Cycle Proteins -- 18 Purification of Cell Cycle Kinases -- 19 Interaction-Based Cloning of Novel Proteins -- 20 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses -- 21 Cell Cycle-Regulated Transcription Factors: Bandshift Assays -- 22 Immunoprecipitation and Immunoblotting in Cell Cycle Studies -- 23 Protein mapping in Cell Cycle Studies -- 24 From Peptide to Purified Antibody.
Keywords: Cell biology; Microbiology; Cancer research; Biochemistry; Animal physiology; Cell Biology; Microbiology; Cancer Research; Biochemistry, general; Animal Physiology