Membrane Proteins Isolation and Characterization - Azzi, Angelo; Masotti, Lanfranco; Vecli, Arnaldo; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1986)
I. Membrane Proteins: Function, Purification, and Characterization -- Purification of Cytochrome c Reductase and Oxidase by Affinity Chromatography -- Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradient Centrifugation -- The Problem of Light Scattering in the Absorbance and Fluorescence Studies of Proteins in Membranes -- Reproducible Preparation of Phosphatidylserine Vesicles for Fluorescence Studies of Protein Incorporation into Lipids -- Purification and Reconstitution of the Proton Translocating Membrane Bound Inorganic Pyrophosphatase from Rhodospirillum rubrum -- The Function of Transmembrane Channels: Ion Transport Studies by 23Na NMR -- Membrane Signal Transduction via Protein Kinase C -- Extraction, Partial Purification, and Reconstitution of a Mixture of Carriers from the Inner Mitochondrial Membrane -- II. Lipid-Protein Interaction -- The Effect of Polypeptides on the Fluidity of Lipid Bilayers -- Polypeptide-Lipid Interactions as Studied by 13C NMR -- Conformational Changes in Polypeptides and Proteins Brought About by Interactions with Lipids -- III. Protein Modification -- Two Examples of Selective Fluorescent Labeling of SH-Groups with Eosin-5-Maleimide: The ADP/ATP Translocator and the Cytochrome c Oxidase Subunit III of Bovine Heart Mitochondria -- Hydrophobic Photolabeling with 125I-TID of Red Blood Cell Membranes -- Use of Fluorescent Probes of the Adenine Nucleotide Carrier for Binding Studies and Analysis of Conformational Changes -- Spin Labeling of Membranes and Membrane Proteins -- IV. Protein Reconstitution -- Functional Reconstitution of the Mitochondrial Cytochrome b-c1 Complex: Effect of Cholesterol -- Changes of the Membrane Surface Potential Measured by Amphiphilic Fluorescent and ESR Probes -- Reconstitution of Cytochrome c Oxidase -- Measurement of Pyruvate Transport in Mitochondria.
Keywords: Biochemistry; Biophysics; Biological physics; Biochemistry, general; Biological and Medical Physics, Biophysics