Pollen Biology A Laboratory Manual - Shivanna, K.R.; Rangaswamy, N.S.; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1992)
1 Introduction -- 2 Pollen Collection -- 3 In Vitro Germination Methods -- Controlled Hydration/Prehydration -- Culture Medium -- Fixation of Pollen Cultures -- Scoring of Pollen Cultures -- General Precautions -- Methods -- Exercise 1 Hanging Drop Culture -- Exercise 2 Sitting Drop Culture -- Exercise 3 Suspension Culture -- Exercise 4 Surface Culture -- Exercise 5 Estimation of Pollen Tube Growth by the Alcian Blue Method -- 4 Pollen Germination and Pollen Tube Growth in Vitro -- Exercise 6 Effects of Boron and Calcium on Pollen Germination and Pollen Tube Growth -- Exercise 7 Leaching of Pollen Substances in Vitro -- Exercise 8 Regulation of pH by Pollen Leachate -- Exercise 9 Role of Transcription and Translation in Pollen Germination and Pollen Tube Growth -- 5 Tests for Pollen Viability -- Exercise 10 In Vitro Germination Test -- Exercise 11 Tetrazolium Test -- Exercise 12 Fluorochromatic Reaction (FCR) Test -- Exercise 13 Fruit Set and Seed Set Test -- Exercise 14 In Vivo Germination and Pollen Tube Growth -- 6 Pollen Storage -- Exercise 15 Effects of Temperature and RH on Viability of Stored Pollen -- Exercise 16 Storage of Pollen Grains in Organic Solvents -- 7 Pollen-Pistil Interaction -- Exercise 17 Cytochemical Localization of Esterases on Stigma Surface -- Exercise 18 Aniline Blue Fluorescence Method to Study Pollen Germination and Pollen Tube Growth in the Pistil -- Exercise 19 Multiple Staining for Localizing Pollen Tubes in the Pistil -- Exercise 20 Semi-Vivo Technique to Study Pollen Germination and Pollen Tube Growth -- Exercise 21 Use of DNA Fluorochromes for Studies on Pollen Grain and Pollen Tube Nuclei -- Exercise 22 Controlled Pollinations -- 8 Self-Incompatibility -- Exercise 23 Confirmation of Self-Incompatibility -- Exercise 24 Studies on Heteromorphic Self-Incompatibility -- Exercise 25 Bud Pollination and Delayed Pollination to Overcome Self-Incompatibility -- Exercise 26 In Vitro Placental Pollination and Fertilization -- 9 Isolation of Protoplasts -- Exercise 27 Isolation of Microspore Protoplasts -- Exercise 28 Isolation of Protoplasts from Pollen Grains -- Exercise 29 Isolation of Sperm Cells (Male Gametes) -- Exercise 30 Isolation of Embryo Sacs and Protoplasts from Embryo Sacs -- 10 Appendices -- Appendix 1 Basic Requirements for Laboratory Exercises -- Appendix 2 Suitable Plant Systems -- Appendix 3 Pollen Morphology -- Appendix 4 Pollen Counts -- Appendix 5 Methods to Obtain Constant Relative Humidity -- Appendix 6 Principles of Fluorescence Microscopy -- Appendix 7 Commonly Used Fluorochromes, and Their Excitation and Fluorescence Ranges -- Appendix 8 Filter Combinations Commonly Used for Different Excitation Ranges -- Appendix 9 Basic Statistical Analyses -- References.
Keywords: Agriculture; Plant science; Botany; Zoology; Forestry; Agriculture; Plant Sciences; Zoology; Forestry