Nucleic Acids in the Environment - Trevors, Jack T.; Elsas, J.Dick van.; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1995)
1 Introduction to Nucleic Acids in the Environment: Methods and Applications -- 1.1 Introduction -- 1.2 Cell Lysis, Nucleic Acid Extraction and Purification Protocols -- 1.3 Analysis of Environmental Nucleic Acids -- 1.4 Concluding Remarks -- References -- 2 Recovery of Bacterial Cells from Soil -- 2.1 Introduction -- 2.2 Strategies for Cell Extraction -- 2.3 Recommended Methods -- 2.4 Practical Experiences and Recommendations -- References -- 3 Extraction, Purification, and Analysis of DNA from Soil Bacteria -- 3.1 Introduction -- 3.2 Bacterial DNA -- 3.3 Direct Extraction of DNA from Soil -- 3.4 Purity Control and Characterization of Soil Bacterial DNA -- References -- 4 Extraction and Analysis of Microbial DNA from Soil -- 4.1 Introduction -- 4.2 DNA Extraction Methods -- 4.3 Evaluation of DNA Extraction Methods -- 4.4 Methods for Analysis of DNA from Soil -- 4.5 Concluding Remarks -- References -- 5 Extraction and Amplification of DNA from the Rhizosphere and Rhizoplane of Plants -- 5.1 Introduction -- 5.2 Sample Preparation -- 5.3 Extraction, Purification and Quantification of DNA from the Rhizosphere -- 5.4 PCR Analysis of Bacterial DNA from the Rhizosphere and Rhizoplane of Plants -- 5.5 Concluding Remarks -- References -- 6 Extraction of DNA from the Phyllosphere -- 6.1 Introduction -- 6.2 Materials, Methods and Protocols -- 6.3 Concluding Remarks -- References -- 7 Specific DNA Sequences for Detection of Soil Bacteria -- 7.1 Introduction -- 7.2 Objectives and Limitations -- 7.3 Target DNA, DNA Extraction and Detection in Soil -- 7.4 Detailed Procedures -- 7.5 Application of Methodology -- References -- 8 PCR Amplification of DNA from Root Nodules -- 8.1 Introduction -- 8.2 Methods for Detection of Rhizobia -- 8.3 Detection of Root Nodule Bacteria by PCR -- 8.4 Conclusions -- References -- 9 Extraction of DNA and RNA from Aquatic Environments -- 9.1 Introduction -- 9.2 DNA as a Biomass and Ecological Indicator -- 9.3 RNA as a Biomass and Ecological Indicator -- 9.4 Biomass and Activity Measurements of Aquatic Microorganisms using RNA and RNA /DNA Ratios -- 9.5 Extraction of Nucleic Acids for Molecular Biological Studies -- 9.6 Molecular Methods for Studying RNA in Aquatic Environments -- 9.7 Extraction of DNA from Aquatic Microbial Populations -- 9.8 Extraction of RNA from Aquatic Microbial Populations -- References -- 10 PCR Amplification of DNA Recovered from the Aquatic Environment -- 10.1 Introduction -- 10.2 Concentration of Water Samples from Aquatic Environments -- 10.3 Sample Preparation for PCR Amplification -- 10.4 Processing of Dissolved DNA in the Aquatic Environment -- 10.5 Processing of Nucleic Acids from Biofilms -- 10.6 PCR Amplification -- 10.7 Identification and Analysis of the Amplified DNA -- 10.8 PCR Contamination Control -- 10.9 Enhancing the Specificity of the PCR Reaction -- 10.10 Use of Arbitrarily Primed PCR (Ap-PCR) in Environmental Microbiology -- 10.11 In situ PCR Amplification -- 10.12 The Use of PCR in Aquatic Environments -- 10.13 Detection of Viruses in Water -- 10.14 Discussion -- References -- 11 Extraction and Amplification of 16S rRNA Genes from Deep Marine Sediments and Seawater to Assess Bacterial Community Diversity -- 11.1 Introduction -- 11.2 DNA Extraction -- 11.3 Amplification of 16S Ribosomal RNA Gene Sequences -- 11.4 Cloning and Sequencing -- 11.5 Sequences Obtained from Marine Sediments and Coastal Waters -- References -- 12 Application of the PCR for Detection of Antibiotic Resistance Genes in Environmental Samples -- 12.1 Introduction -- 12.2 Selection of PCR Primers and Gene Probes for Specific Detection of sat and nptII Genes -- 12.3 Detection of Antibiotic Resistance Genes in Bacterial Isolates Using PCR -- 12.4 Detection of Antibiotic Resistance Genes Using DNA Extraction and PCR -- 12.5 Detection of Antibiotic Resistance Genes in Total Community DNA Extracts from Environmental Habitats. -- 12.6 Conclusions -- References.
Keywords: Geography; Geoecology; Environmental geology; Nature conservation; Geography, general; Geoecology/Natural Processes; Nature Conservation