Microinjection and Transgenesis Strategies and Protocols - Cid-Arregui, Angel; Garcia-Carranca, Alejandro; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1998)
1 Strategies and Protocols for Microinjection Experiments Using Somatic Cells in Culture -- 1 Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells -- 2 Automated Computer-Assisted Microinjection into Cultured Somatic Cells -- 3 Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells -- 4 Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture -- 5 Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Experiments -- 6 Colorimetric in Situ Hybridization -- 7 Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tissue Culture Cells -- 8 Cellular Microbiochemistry -- 9 A New Strategy for Studying Microtubule Transport and Assembly During Axon Growth -- 10 The Use of Microinjection to Study Signal Transduction in Mammalian Cells -- 2 Strategies and Protocols for Microinjection Experiments Using Embryos -- 11 Supply and Husbandry of Mice for Transgenic Science -- 12 Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis? -- 13 Sperm-Mediated Gene Transfer -- 14 Application of Ligand-Dependent Site-Specific Recombination in ES Cells -- 15 A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryos -- 16 The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem Cells -- 17 The Generation of Transgenic Mice with Yeast Artificial Chromosomes -- 18 Following Up Microinjected mRNAs: Analysis of mRNA Poly(A) Tail Length During Mouse Oocyte Maturation -- 19 Mouse Preimplantation Embryos and Oocytes as an In Vivo System to Study Transcriptional Enhancers -- 20 The Use of Mouse Preimplantation Embryos for the Identification of DNA-Binding Proteins Essential in Early Development -- 21 Application of Transgenes and Transgenic Mice to Study Gene Activity from the Oocyte to the Early Embryo -- 22 Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements -- 23 Transgenic Analysis of Embryonic Gene Expression Using LacZ as a Reporter -- 24 Autonomous Cell Labeling Using a laacZ Reporter Transgene to Produce Genetic Mosaics During Development -- 25 Multiple Developmental Functions of the Zinc Finger Gene Krox-20 Are Revealed by Targeted Insertion of the E. coli lacZ Coding Sequence -- 26 Transgenic Mouse Strategies in Virus Research -- 27 Isolation of Mutated Genes from Transgene Insertion Sites -- 28 Contributions of Transgenic and Knockout Mice to Immunological Knowledge -- 29 Generation and Application of Transgenic Rabbits -- 30 Microinjection of Drosophila Eggs.
Keywords: Cell biology; Cell Biology