Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods - McGenity, Terry J.; Timmis, Kenneth N.; Nogales, Balbina; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2016)
Introduction: Single cell biotechnology for uncultured microorganisms -- Single-cell cultivation in microfluidic devices -- Single-cell bacterial bioreporter assays to measure hydrocarbons -- Whole genome amplification of single bacterial cells -- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria -- Localisation of proteins within intact bacterial cells using fluorescent protein fusions -- Raman-FISH -- Single-cell metabolomics -- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division -- Single cell biotechnology for uncultured microorganisms -- Single cell and systems biology tools for biofuel production -- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) -- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity -- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence. .
Keywords: Microbiology; Biochemical engineering; Microbiology; Biochemical Engineering