Hydrocarbon and Lipid Microbiology Protocols Genetic, Genomic and System Analyses of Communities - McGenity, Terry J.; Timmis, Kenneth N.; Nogales, Balbina; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2017)
Introduction to genetic, genomic and system analyses for communities -- Genomic analysis of pure cultures and communities -- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems -- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems -- Prokaryotic metatranscriptomics -- Defining a pipeline for metaproteomic analyses -- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing -- DNA and RNA-based stable isotope probing of hydrocarbon degraders -- Protein stable isotope probing (Protein-SIP) -- Protein extraction from contaminated soils and sediments -- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities -- Physiological modelling of complex bacterial communities -- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants.
Keywords: Microbiology; Biochemical engineering; Microbiology; Biochemical Engineering