Non-viral Gene Therapy Gene Design and Delivery - Taira, Kazunari; Kataoka, Kazunori; Niidome, Takuro; SpringerLink (Online service) (Tokyo : Springer Japan : Imprint: Springer, 2005)
Molecules for Gene Therapy -- Recent Progress in Non-viral Gene Delivery -- Physical Chemistry of DNA-Carrier Complexes -- Liposomes -- Polymeric Gene Carriers -- Development of a Supramolecular Nanocarrier for Gene Delivery Based on Cationic Block Copolymers -- Chitosan -- Dendrimers as DNA Carriers -- Use of Synthetic Peptides for Non-viral Gene Delivery -- An Oligonucleotide Carrier Based on ?-1,3-Glucans -- Biological and Chemical Hybrid Vectors -- Controlled Gene Delivery -- Pharmacokinetics of Gene Delivery in Cells -- Active DNA Release from Complexes -- Controlled Release of DNA Using Thermoresponsive Polymers -- Active Transport of Exogenous Genes into the Nucleus -- Controlled Intracellular Localization of Oligonucleotides by Chemical Conjugation -- Functional Nucleotide Sequences Capable of Promoting Non-viral Genetic Transfer -- Shielding of Cationic Charge of the DNA Complex to Avoid Nonspecific Interactions for In Vivo Gene Delivery -- In Vivo Gene Transfer by Ligand-Modified Gene Carriers -- Optimizing Polyplexes into Synthetic Viruses for Tumor-Targeted Gene Therapy -- Gene Transfer and Target Diseases -- Clinical Trials Using Non-viral Gene Delivery Systems -- Current Protocols of Gene Delivery -- Evaluation of Size and Zeta Potential of DNA/Carrier Complexes -- Observation of DNA/Carrier Complexes Under Fluorescence Microscopy -- Synthesis of Chemically Modified Chitosan and a Study of Its Gene Transfection Efficiency -- Newly Designed DNA Fragments for Gene Correction -- Evaluation of Gene Expression In Vivo After Intravenous and Intraportal Administration of Lipoplexes -- Evaluation by Southern Blot Hybridization of DNA Administered with a Gene Carrier to Organs -- Evaluation of the Immune Response After Administration of Plasmid DNA-Non-viral Vector Complexes -- Gateway RNAi -- Design of Genes Based on Current RNA Technology -- Design of Intracellularly Active Ribozymes and siRNAs -- RNAi-Based Inhibition Specific for Mutant Alleles in Autosomal Dominant Diseases: Sequence-Dependent and -Independent Discrimination of Mutant and Wild-Type Alleles by siRNA -- In Vivo RNA Interference: Another Tool in the Box? -- Suppression of Gene Expression via Chromatin Remodeling and the siRNA-Induced Silencing of Transcription -- Intracellular Delivery of Nucleic Acids: Differences Between Transfection and siFection Reflect Differences Between DNA and RNA, and Between Oligodeoxynucleotides and Oligonucleotides -- In Vivo Antitumor Activity of a New Cationic Liposome siRNA Complex -- Enhancing RNAi with Synthetic RNA Duplexes.
Keywords: Cell biology; Biotechnology; Molecular biology; Genetic engineering; Biochemical engineering; Medical biochemistry; Cell Biology; Biotechnology; Molecular Medicine; Genetic Engineering; Biochemical Engineering; Medical Biochemistry