Plant Tissue Culture Manual Supplement 6 - Lindsey, K.; SpringerLink (Online service) (Dordrecht : Springer Netherlands : Imprint: Springer, 1996)
Section A: Basic Techniques - Cell & Tissue Culture of Model Species -- 1. Media preparation -- 2. The initiation and maintenance of callus cultures of carrot & tobacco -- 3. The initiation and maintenance of plant cell suspension cultures -- 4. Shoot cultures and root cultures of tobacco -- 5. Somatic embryogenesis in orchardgrass -- 6. Arabidopsis regeneration and transformation (leaf & cotyledon expiant system) -- 7. Arabidopsis protoplast transformation and regeneration -- 8. Arabidopsis regeneration and transformation (root expiant system) -- 9. Somatic embryogenesis in carrot -- 10. Low density cultures: microdroplets and single-cell nurse cultures -- 11. Tobacco protoplast isolation, culture and regeneration -- Section B: Tissue Culture & Transformation of Crop Species -- 1. Embryogenic callus, cell suspension and protoplast cultures of cereals -- 2. Transformation and regeneration of rice protoplasts -- 3. Transformation and regeneration of orchardgrass protoplasts -- 4. Transformation and regeneration of oilseed rape protoplasts -- 5. Regeneration and transformation of potato by Agrobacterium tumefaciens -- 6. Transformation of tomato with Agrobacterium tumefaciens -- 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens -- 8. Regeneration and transformation of apple (Malus pumila Mill.) -- 9. Transformation and regeneration of maize protoplasts -- 10. Regeneration and transformation of barley protoplasts -- 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation -- 12. Production of fertile transgenic wheat by microprojectile bombardment -- 13. Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment -- Section C: Propagation & Conservation of Germplasm -- 1. Clonal propagation of orchids -- 2. Clonal propagation of palms -- 3. Clonal propagation of conifers -- 4. Cytological techniques -- 5. Restriction fragment analysis of somaclones -- 6. Virus elimination and testing -- 7. Clonal propagation of Citrus -- 8. Clonal propagation of eucalypts -- 9. Cryopreservation of plant tissue cultures: the example of embryogénie tissue cultures from conifers -- Section D: Direct Gene Transfer & Protoplast Fusion -- 1. Gene transfer by particle bombardment -- 2. Transformation of pollen by particle bombardment -- 3. Electrical fusion of protoplasts -- 4. Cybrid production and selection -- 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids -- 6. RFLP analysis of organellar genomes in somatic hybrids -- 7. Isolation and uptake of plant nucle -- 8. In situ hybridization to plant metaphase chromosomes: Radioactive and non-radioactive detection of repetitive and low copy number genes -- 9. Chemical fusion of protoplasts -- Section E: Reproductive Tissues -- 1. In vitro fertilisation of maize -- 2. Endosperm culture -- 3. Endosperm culture -- 4. Hybrid embryo rescue -- 5. In vitro culture of Brassica juncea zygotic proembryos -- Section F: Mutant Selection -- 1. Use of chemical and physical mutagens in vitro -- 2. In vitro culture, mutant selection, genetic analysis and transformation of Physcomitrella patens -- Section G: Secondary Metabolites -- 1. Tropane alkaloid biosynthesis in vitro -- 2. Anthocyanin biosynthesis in vitro -- 3. Biosynthesis of monoterpene indole alkaloids in vitro -- Section H: Tissue Culture Techniques for Fundamental Studies -- 1. Establishment of photoautotrophic cell cultures -- 2. Zinnia mesophyll culture system to study xylogenesis -- 3. Cell cycle studies: induction of synchrony in suspension cultures of Catharanthus roseus cells H. Kodama -- 4. Thin Cell Layer (TCL) method to programme morphogenetic patterns -- 5. In vitro infection of Arabidopsis with nematodes.
Keywords: Plant science; Botany; Cell biology; Plant Sciences; Cell Biology