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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a REIFFERS, ISABELLE
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| 245 |
1 |
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|a PRODUCTION OF DOUBLED HAPLOID RICE PLANTS (ORYZA SATIVA L.) BY ANTHER CULTURE.
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| 520 |
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|a The results of anther culture of F1 pollen issued from 23 single crosses are presentes. A relation between the morphology of the panicle and the microspore stage was established. After cold-pretreatment (8 days at 4ºC), the anthers were cultured on the callus-induction medium N6 supplemented with 1 mg 1 - 1 naphthaleneacetic acid. The calli were transferred to MS plant regeneration medium supplemented with 3mgl-1 kinetin + 0.5 mg l-1 naphthaleneacetic acid. The induction frequency varied from 0.22% to 29% and the regeneration frequency from 0% to 144.4%, dependent upon the crosses used. On average, 27% of the plants obtained were albinos and 59% of the green plants underwent spontaneous chromosome doubling, Thirty- nine doubled haploid lines were evaluated and multiplied in the field. Lines with an excellent behaviour in upland culture conditions were selected from two crosses.
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| 650 |
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4 |
|a ARROZ
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| 650 |
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4 |
|a ORYZA SATIVA
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| 650 |
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4 |
|a HAPLOIDA
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| 650 |
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4 |
|a CULTIVO DE ANTERAS Y TEJIDOS
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| 650 |
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4 |
|a BIOTECNOLOGIA
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| 655 |
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4 |
|a Artículos de revista
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| 700 |
1 |
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|a ADELSON B. FREIRE
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| 773 |
1 |
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|t PLANT CELL, TISSUE AND ORGAN CULTURE
|g Vol. 21, núm.2 (mayo, 1990), páginas 165-170
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| 905 |
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|a BSC_ANA
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