Calcium Signalling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Raffaello, Anna. (Editor ), Vecellio Reane, Denis. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1925
Materias:
Tabla de Contenidos:
  • High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels
  • Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling
  • Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators
  • Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes
  • MCU Regulation in Lipid Bilayer and Electrophysiological Recording
  • Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer
  • Patch Clamp Analysis of the Mitochondrial Calcium Uniporter
  • In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana
  • Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes
  • Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes
  • In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle
  • TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis
  • Ca2+-Dependent Regulation of TFEB and Lysosomal Function
  • Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum
  • Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2
  • Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes
  • Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes
  • Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer
  • Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells
  • Purification of Functional F-ATP Synthase from Blue Native PAGE.