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170111s2017 xxu| s |||| 0|eng d |
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|a 9781493967803
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| 024 |
7 |
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|a 10.1007/978-1-4939-6780-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a CD95 :
|b Methods and Protocols /
|c edited by Patrick Legembre.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XI, 230 p. 63 illus., 38 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1557
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| 505 |
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|a Production of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L -- CD95 Stimulation with CD95L and DISC Analysis -- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8 -- In vitro Evaluation of the Apoptosis Function in Human Activated T cells -- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition -- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization -- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins -- CD95-mediated Calcium Signaling -- CD95-mediated Proton Regulation -- Study of the CD95-mediated Non-apoptotic Signaling Pathway – PI3K -- Organelle Separation and Cell Signaling -- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L -- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions -- Quantifying CD95/cl-CD95L Implications in Cell Mechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements -- Sketching of CD95 Oligomers by In Silico Investigations -- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE -- Detection of S-acylated CD95 by Acyl-biotin Exchange -- Exploration of Fas S-nitrosylation by the Biotin Switch Assay -- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L -- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L -- CD95 and the MRL-lpr Mouse Model.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Legembre, Patrick.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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