Chromatin Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Chellappan, Srikumar P. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:3rd ed. 2015.
Series:Methods in Molecular Biology, 1288
Subjects:
Table of Contents:
  • Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support
  • Preparation and Analysis of Positioned Mononucleosomes
  • Chromatin Imaging with Time-Lapse Atomic Force Microscopy
  • Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR
  • Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin
  • Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes
  • Histone Deacetylase Activity Assay
  • In Vitro Histone Demethylase Assays
  • Integrated DNA Methylation and Chromatin Structural Analysis at Single-Molecule Resolution
  • Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips
  • Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction
  • In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
  • Immunostaining Analysis of Tissue Cultured Cells and Tissue Sections Using Phospho-Histone H3 (Serine 10) Antibody
  • Identification and Characterization of Nonhistone Chromatin Proteins: Human Positive Coactivator 4 as a Candidate
  • Methods to Study Transcription-Coupled Repair in Chromatin
  • Analysis of DNA Replication Associated Chromatin Decondensation: In Vivo Assay for Understanding Chromatin Remodeling Mechanisms of Selected Proteins
  • Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
  • Detecting ATM Dependent Chromatin Modification in DNA Damage Response
  • Imaging Local Deposition of Newly Synthesized Histones in UVC-Damaged Chromatin
  • In Vitro Replication Assay with Mammalian Cell Extracts
  • Fluorescent In Situ Hybridization on Comets: FISH Comet
  • Methods to Study Histone Chaperone Function in Nucleosome Assembly and Chromatin Transcription
  • Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase Using spFRET
  • Transcriptome-Wide Identification of In Vivo Interactions Between RNAs and RNA-Binding Proteins by RIP and PAR-CLIP Assays
  • Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo
  • ChIP on Chip and ChIP-Seq Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications
  • ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays.