Calcium-Binding Protein Protocols : Volume 2: Methods and Techniques /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vogel, Hans J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edición:1st ed. 2002.
Colección:Methods in Molecular Biology, 173
Materias:
Tabla de Contenidos:
  • Methods and Techniques to Study
  • Quantitative Analysis of Ca2+-Binding by Flow Dialysis
  • Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators
  • Deconvolution of Calcium-Binding Curves
  • Absorption and Circular Dichroism Spectroscopy
  • Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins
  • Steady-State Fluorescence Spectroscopy
  • Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins
  • Surface Plasmon Resonance of Calcium-Binding Proteins
  • Differential Scanning Calorimetry
  • Isothermal Titration Calorimetry
  • Multiangle Laser Light Scattering and Sedimentation Equilibrium
  • Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets
  • Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry
  • Synthetic Calcium-Binding Peptides
  • Proteolytic Fragments of Calcium-Binding Proteins
  • Electron Magnetic Resonance Studies of Calcium-Binding Proteins
  • Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins
  • Calcium-43 NMR of Calcium-Binding Proteins
  • Exploring Familial Relationships Using Multiple Sequence Alignment
  • Structure Determination by NMR
  • Protein Structure Calculation from NMR Data
  • Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation
  • The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains
  • Vector Geometry Mapping
  • Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography
  • Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras
  • Gene Expression inTransfected Cells
  • Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins
  • Studying the Spatial Distribution of Ca2+-Binding Proteins.