Bacterial Toxins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Holst, Otto. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Edición:1st ed. 2000.
Colección:Methods in Molecular Biology, 145
Materias:
Tabla de Contenidos:
  • Bacterial Protein Toxins
  • Purification of Clostridium botulinum Type A Neurotoxin
  • Shiga Toxins
  • Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins
  • Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins
  • Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins
  • Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins
  • The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins
  • Site-Directed Spin Labeling of Proteins
  • Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers
  • Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance
  • ADP-Ribosylation of ?-Gi Proteins by Pertussis Toxin
  • Phage Libraries for Generation of Anti-Botulinum scFv Antibodies
  • T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors
  • In Vitro Physiological Studies on Clostridial Neurotoxins
  • The Biology of Endotoxin
  • Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides
  • Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides
  • Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates
  • Electrophysiological Measurements on Reconstituted Outer Membranes.