Bacterial Artificial Chromosomes : Volume 2: Functional Studies /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Zhao, Shaying. (Editor ), Stodolsky, Marvin. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 256
Materias:
Tabla de Contenidos:
  • Use of BAC End Sequences for SNP Discovery
  • Exon Trapping for Positional Cloning and Fingerprinting
  • Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column
  • BAC Microarray-Based Comparative Genomic Hybridization
  • Large DNA Transformation in Plants
  • Retrofitting BACs With a Selectable Marker for Transfection
  • BAC Modification Using a RecA Expressing Shuttle Vector System
  • Bacterial Artificial Chromosome Engineering
  • ET Recombination
  • BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes
  • Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes
  • Generation of BAC Transgenic Mice
  • BAC Rescue
  • Herpesviruses
  • Cloning of ?-Herpesvirus Genomes as Bacterial Artificial Chromosomes
  • Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors
  • Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination)
  • Mutagenesis of Herpesvirus BACs by Allele Replacement
  • Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies
  • Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I.