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100301s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597455237
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| 024 |
7 |
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|a 10.1007/978-1-59745-523-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Auditory and Vestibular Research :
|b Methods and Protocols /
|c edited by Bernd Sokolowski.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XII, 428 p. 61 illus., 13 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 493
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| 505 |
0 |
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|a Nucleic Acid Protocols -- RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning -- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays -- In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections -- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification -- Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus -- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research -- Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epithelium -- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear -- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear -- Molecular Biology of Vestibular Schwannomas -- Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens -- Fluorescence “In Situ” Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa -- Positional Cloning of Deafness Genes -- Amino Acid Protocols -- Twist-Off Purification of Hair Bundles -- Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System -- The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea -- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis -- In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation -- Identification of Transcription Factor–DNA Interactions Using Chromatin Immunoprecipitation Assays -- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins in Inner-Ear Sensory Epithelia -- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research -- Imaging Protocols -- Fluorescence Microscopy Methods in the Study of Protein Structure and Function -- Ion Imaging in the Cochlear Hair Cells -- Atomic Force Microscopy in Studies of the Cochlea.
|
| 650 |
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0 |
|a Human physiology.
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| 650 |
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0 |
|a Neurology .
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| 650 |
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0 |
|a Molecular biology.
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| 650 |
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0 |
|a Pathology.
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| 650 |
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0 |
|a Gene expression.
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| 650 |
1 |
4 |
|a Human Physiology.
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| 650 |
2 |
4 |
|a Neurology.
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| 650 |
2 |
4 |
|a Molecular Medicine.
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| 650 |
2 |
4 |
|a Pathology.
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| 650 |
2 |
4 |
|a Gene Expression.
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| 700 |
1 |
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|a Sokolowski, Bernd.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|