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160203s2016 xxu| s |||| 0|eng d |
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|a 9781493933938
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| 024 |
7 |
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|a 10.1007/978-1-4939-3393-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Biotechnology of Plant Secondary Metabolism :
|b Methods and Protocols /
|c edited by Arthur Germano Fett-Neto.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XI, 180 p. 37 illus., 22 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1405
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| 505 |
0 |
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|a Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal -- Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction -- A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines -- Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves -- From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate : Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica -- Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli -- Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala -- Production of Aromatic Plant Terpenoids in Recombinant Baker´s Yeast -- Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) -- A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes -- Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters -- Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants -- Protoplast Transformation as a Plant-Transferable Transient Expression System -- Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System -- Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
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| 650 |
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|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Fett-Neto, Arthur Germano.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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