Calcium Signaling Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lambert, David G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2005.
Edición:2nd ed. 2005.
Colección:Methods in Molecular Biology, 312
Materias:
Tabla de Contenidos:
  • General
  • Fluorescent Measurement of [Ca2+]c
  • Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
  • Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
  • Specialist Measurement Systems
  • Confocal Microscopy
  • Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration
  • Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer
  • Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
  • Measurement of Ca2+ Channel Activity
  • Measurement of Ca2+ Entry Using 45Ca2+
  • Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding
  • Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents
  • Measurement of Ins(1,4,5,)P3 and Ca2+ Release From Intracellular Stores
  • Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells
  • Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
  • Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay
  • Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells
  • Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
  • Specialist Measurement Techniques
  • Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
  • Measurement of Calcium and Movement in Heart Cells
  • Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations
  • Measurement of Cytosolic-Free Ca2+ in Plant Tissue
  • Ca2+-Sensitive Targets
  • Assay and Purification of Calmodulin-Dependent Protein Kinase
  • Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
  • Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).