Basic Cell Culture Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Helgason, Cheryl D. (Editor ), Miller, Cindy L. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:4th ed. 2013.
Colección:Methods in Molecular Biology, 946
Materias:
Tabla de Contenidos:
  • 1. Detection of Mycoplasma Contaminations
  • 2. Eradication of Mycoplasma Contaminations
  • 3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination
  • 4. Classical and Molecular Cytogenetic Analysis
  • 5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle
  • 6. The Development of T Lymphocytes in Fetal Thymus Organ Culture
  • 7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
  • 8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells
  • 9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media
  • 10. Isolation and Characterization of Mouse Side Population Cells
  • 11. Stem Cell Identification by DyeCycle Violet Side Population Analysis
  • 12. Isolation and Characterization of Cancer Stem Cells In Vitro
  • 13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets
  • 14. Generation and Characterization of Murine Alternatively Activated Macrophages
  • 15. Human Long Term Culture Initiating Cell (LTC-IC) Assay
  • 16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells
  • 17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
  • 18. Studying Leukocyte Recruitment Under Flow Conditions
  • 19. Generation and Establishment of Murine Adherent Cell Lines
  • 20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture
  • 21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone
  • 22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture
  • 23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells
  • 24. Human Prostate Epithelial Cell Cultures
  • 25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue
  • 26. Isolation and Characterization of Human Hair Follicle Epithelial Cells
  • 27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells
  • 28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
  • 29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells
  • 30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System
  • 31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
  • 32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates
  • 33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells.