C. elegans : Methods and Applications /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edition: | 1st ed. 2006. |
| Series: | Methods in Molecular Biology,
351 |
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Table of Contents:
- An Overview of C. elegans Biology
- Comparative Genomics in C. elegans, C. briggsae, and Other Caenorhabditis Species
- WormBase
- C. elegans Deletion Mutant Screening
- Insertional Mutagenesis in C. elegans Using the Drosophila Transposon Mos1
- Single-Nucleotide Polymorphism Mapping
- Creation of Transgenic Lines Using Microparticle Bombardment Methods
- Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA
- Delivery Methods for RNA Interference in C. elegans
- Functional Genomic Approaches in C. elegans
- Assays for Toxicity Studies in C. elegans With Bt Crystal Proteins
- Fluorescent Reporter Methods
- Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans
- Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis
- Preservation of C. elegans Tissue Via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immunocytochemistry
- Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants
- Automated Imaging of C. elegans Behavior
- Intracellular Ca2+ Imaging in C. elegans
- In Vitro Culture of C. elegans Somatic Cells
- Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS™ Flow-Sorting System.