Chloroplast Research in Arabidopsis : Methods and Protocols, Volume II /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Jarvis, R. Paul. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 775
Materias:
Tabla de Contenidos:
  • One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes
  • Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems
  • Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification
  • Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET)
  • Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods
  • Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis
  • Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis
  • Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis
  • Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis
  • Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies
  • Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis
  • Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies
  • Preparation and Proteomic Analysis of Chloroplast Ribosomes
  • The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting
  • Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts
  • Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants
  • Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves
  • Measurement of the ΔpH and Electric Field Developed Across Arabidopsis Thylakoids in the Light
  • Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis
  • Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis
  • Analysis of Starch Metabolism in Chloroplasts
  • The Analysis of Lipid Content and Quality in Arabidopsis Plastids.