CRISPR : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | , , |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edition: | 1st ed. 2015. |
| Series: | Methods in Molecular Biology,
1311 |
| Subjects: |
Table of Contents:
- Investigating CRISPR RNA Biogenesis and Function using RNA-seq
- In vitro Co-reconstitution of Cas Protein Complexes
- Analysis of CRISPR pre-crRNA Cleavage
- Annotation and Classification of CRISPR-Cas Systems
- Computational Detection of CRISPR/crRNA Targets
- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium
- Spacer-based Macroarrays for CRISPR Genotyping
- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS)
- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR
- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli
- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes
- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus
- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids
- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition
- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl
- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates
- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems
- Cas3 Nuclease-Helicase Activity Assays
- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex
- Creation and Analysis of a Virome: Using CRISPR Spacers
- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases
- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9
- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).