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100301s2000 xxu| s |||| 0|eng d |
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|a 9781592590506
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| 024 |
7 |
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|a 10.1385/1592590500
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Calpain Methods and Protocols /
|c edited by John S. Elce.
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| 250 |
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|a 1st ed. 2000.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2000.
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| 300 |
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|a XVIII, 342 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 144
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| 505 |
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|a Purification of Components of the Calpain System -- Purification of µ- and m-Calpain and Calpastatin from Animal Tissues -- A Simple Protocol for Separation and Assay of µ-Calpain, m-Calpain and Calpastatin from Small Tissue Samples -- Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms from Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography -- Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose -- Affinity Purification of ?-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump -- Expression of m-Calpain in Escherichia coli -- Purification and Characterization of Crustacean Calpain-like Proteinases -- Drosophila Cal pains -- Molecular Analysis of p94 and Its Application to Diagnosis of Limb Girdle Muscular Dystrophy Type 2A -- Purification of Recombinant Calpastatin Expressed in Escherichia coli -- Preparation of Calpastatin Samples for Western Blotting -- Isolation and Characterization of Calpain Activator Protein from Bovine Brain -- Assays of Calpain -- Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein -- Casein Zymogram Assessment of ?-Calpain and m-Calpain Activity After Traumatic Brain Injury in the Rat In Vivo -- Fluorescence Measurements of Ca2+ Binding to Domain VI of Calpain -- Kinetic Analysis of Human ?-Calpain Autolysis -- A Sensitive and Continuous Fluorometric Activity Assay Using a Natural Substrate -- Measurement of Calpain Activity In Vitro and In Situ Using a Fluorescent Compound andTau as Substrates -- Localization of Calpain by Immunofluorescence in Adherent Cells -- A Radioimmunologic Technique for Assessing Calpain Activation in Cells -- Specific Tissues -- Calpains and Myogenesis -- Calpastatin (the Endogenous Calpain Inhibitor) and Membrane Protein Degradation in Cell Fusion -- The Role of Calpain in Neurofilament Protein Degradation Associated With Spinal Cord Injury -- Calpain-Mediated Truncation of Glutamate Ionotropic Receptors -- Concurrent Assessment of Calpain and Caspase-3 Activity by Means of Western Blots of Protease-Specific Spectrin Breakdown Products -- Rat Renal Proximal Tubules, Hypoxia, lonomycin, and Calpain -- Calpain Activity in Rat Renal Proximal Tubules -- Calpain Activity in Rat Renal Proximal Tubules -- Cellular In Vivo Assay of Calpain Activity Using a Fluorescent Substrate -- Calpain Methods in Hepatic Ischemia-Reperfusion Injury -- Myocardial Ischemia-Reperfusion Injury and Proteolysis of Fodrin, Ankyrin, and Calpastatin -- Calpains in the Lens and Cataractogenesis -- Specific Substrates -- Proteolysis of Cortactin by Calpain in Platelets and In Vitro -- Proteolysis of p53 Protein by Ubiquitous Calpains -- Modulation of Calpain-Mediated Protein Kinase C Activation Within Intact Cells -- Strategies for Regulating Calpain Activities in Living Cells -- Assays of Apoptosis.
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| 650 |
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|a Neurosciences.
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| 650 |
1 |
4 |
|a Neurosciences.
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| 700 |
1 |
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|a Elce, John S.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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