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131115s2014 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627037150
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| 024 |
7 |
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|a 10.1007/978-1-62703-715-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Cereal Genomics :
|b Methods and Protocols /
|c edited by Robert J. Henry, Agnelo Furtado.
|
| 250 |
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|a 1st ed. 2014.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
|
| 300 |
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|a X, 300 p. 39 illus., 29 illus. in color. :
|b online resource.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
|
| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1099
|
| 505 |
0 |
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|a DNA Extraction from Vegetative Tissue for Next Generation Sequencing -- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) -- RNA Extraction from Cereal Vegetative Tissue -- RNA Extraction from Developing or Mature Wheat Seeds -- cDNA Library Preparation -- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants -- The Polymerase Chain Reaction (PCR) - General Methods -- Mutation and Mutation Screening -- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression -- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel -- Rapid Cloning of Genes and Promoters for Functional Analyses -- Genome Walking -- Functional Analysis by Protein Biochemistry -- Genomic Southern Blot analysis -- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs -- Protein Blotting-Protocol for Beginners -- Genetic Transformation of Wheat via Particle Bombardment -- Sorghum Genetic Transformation by Particle Bombardment -- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery -- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation -- Agrobacterium-mediated Transformation- Rice Transformation -- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos -- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops -- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.
|
| 650 |
|
0 |
|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
|
0 |
|a Plant genetics.
|
| 650 |
|
0 |
|a Transgenic organisms.
|
| 650 |
1 |
4 |
|a Plant Sciences.
|
| 650 |
2 |
4 |
|a Plant Genetics and Genomics.
|
| 650 |
2 |
4 |
|a Transgenics.
|
| 700 |
1 |
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|a Henry, Robert J.
|e editor.
|
| 700 |
1 |
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|a Furtado, Agnelo.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|