Bacterial Cell Surfaces : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Delcour, Anne H. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 966
Materias:
Tabla de Contenidos:
  • A Bird’s Eye View of the Bacterial Landscape
  • Visualizing the Bacterial Cell Surface: An Overview
  • Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems
  • Experimental Manipulation of the Microbial Functional Amyloid Called Curli
  • Visualization of Gram-Positive Bacterial Pili
  • Single Cell Microfluidic Studies of Bacterial Motility
  • Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule
  • Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy
  • Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance
  • Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System
  • Pore Formation by T3SS Translocators: Liposome Leakage Assay
  • Isolation of Bacterial Type IV Machine Subassemblies
  • Production and Crystallization of Bacterial Type V Secretion Proteins
  • Assembly of Bacterial Outer Membrane Proteins
  • The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization
  • Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles
  • In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate
  • Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes
  • Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis
  • Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues
  • Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response
  • Isolation of Bacteria Envelope Proteins
  • Patch Clamp Electrophysiology for the Study of Bacterial Ion Channels in Giant Spheroplasts of E. coli
  • Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid Bilayers.