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170701s2017 xxu| s |||| 0|eng d |
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|a 9781493970339
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| 024 |
7 |
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|a 10.1007/978-1-4939-7033-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Bacterial Protein Secretion Systems :
|b Methods and Protocols /
|c edited by Laure Journet, Eric Cascales.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XV, 528 p. 96 illus., 70 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1615
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| 505 |
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|a Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder -- Protein Sorting Prediction -- Cell Fractionation -- Defining Lipoprotein Localisation by Fluorescence Microscopy -- Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate -- Defining Membrane Protein Localization by Isopycnic Density Gradients -- Components Sub-cellular Localisation: Cell Surface Exposure -- Probing Inner Membrane Protein Topology by Proteolysis -- Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM) -- Defining Membrane Protein Topology using pho-lac Reporter Fusions -- In vivo and in vitro Protein-peptidoglycan Interactions -- Measure of Peptidoglycan Hydrolase Activity -- Protein-protein Interaction: Bacterial Two-Hybrid -- Protein-protein Interactions: Yeast two-hybrid -- Protein-protein Interactions: Cytology Two-hybrid -- Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes -- Protein-protein Interactions: Co-immunoprecipitation -- Protein-protein Interaction: Tandem Affinity Purification in Bacteria -- Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes -- Protein-protein Interactions: Pull-down Assays -- Protein-protein interactions: Surface Plasmon Resonance -- Assessing Energy-dependent Protein Conformational Changes in the TonB System -- Defining Assembly Pathways by Fluorescence Microscopy -- Large Complexes: Cloning Strategy, Production, and Purification -- Shearing and Enrichment of Extracellular Type IV Pili -- Blue Native PAGE Analysis of Bacterial Secretion Complexes -- In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography -- Structural Analysis of Protein Complexes by Cryo Electron Microscopy -- Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy -- Identification of Effectors: Precipitation of Supernatant Material -- Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System -- Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot -- Effector Translocation: Cya Reporter Assay -- Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System -- Effector Translocation Assay: Differential Solubilization -- Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture. .
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 700 |
1 |
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|a Journet, Laure.
|e editor.
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| 700 |
1 |
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|a Cascales, Eric.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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