C. elegans : Methods and Applications /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Biron, David. (Editor ), Haspel, Gal. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:2nd ed. 2015.
Colección:Methods in Molecular Biology, 1327
Materias:
Tabla de Contenidos:
  • Library Construction for Mutation Identification by Whole-Genome Sequencing
  • Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes
  • Genetic Methods for Cellular Manipulations in C. elegans
  • A Fusion PCR Method for Expressing Genetic Tools in C. elegans
  • Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI)
  • Creating Genome Modifications in C. elegans using the CRISPR/Cas9 System
  • Observing and Quantifying Fluorescent Reporters
  • Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior
  • Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans
  • High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans
  • Electron Tomography Methods for C. elegans
  • Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
  • Tracking Single C. elegans using a USB Microscope on a Motorized Stage
  • An Imaging System for C. elegans Behavior
  • A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae
  • Sampling and Isolation of C. elegans from the Natural Habitat
  • A Primer on Prototyping
  • A Primer on Quantitative Modeling.