Cell-Free Protein Synthesis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Alexandrov, Kirill. (Editor ), Johnston, Wayne A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1118
Materias:
Tabla de Contenidos:
  • Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae
  • Bioinformatics Analysis and Optimization of Cell-Free Protein Expression
  • A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression
  • One-Pot, Microscale Cell-Free Enzyme Expression and Screening
  • Cell-Free Translation of Biofuels Enzymes
  • Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems
  • High Level Cell-Free Production of Membrane Proteins with Nanodiscs
  • Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study
  • Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis
  • Cell-Free Protein Synthesis in Microfluidic 96-Well Plates
  • Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis
  • Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis
  • In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate
  • An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA
  • In vitro Translation Based Protein Kinase Substrate Identification
  • Preparation of Protein Arrays using Cell-Free Protein Expression
  • Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System
  • Cell-Free Membrane Protein Expression
  • The PURE System for Protein Production
  • A Cell-Free Protein Synthesis System from Insect Cells
  • A Cell-Free Expression Platform for Production of Protein Microarrays.