G Protein-Coupled Receptor Signaling : Methods and Protocols /
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1947 |
| Materias: |
Tabla de Contenidos:
- Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics
- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs
- Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies
- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties
- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions
- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands
- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs
- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology
- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches
- Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays
- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions
- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters
- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays
- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay
- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry
- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy
- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies
- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy
- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy
- Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug
- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice
- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents
- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs.